DNA Quantifier
A simple and straightforward method has been developed by a cross-division team at the Los Alamos National Laboratory for the quantitation of DNA in soil and sediment samples. This method has been used to develop a prototype of a field-portable custom spectrometer that combines fluorescence and absorption spectroscopy to perform the analysis. This instrument competes with bench top instrumentation in terms of detection limit and sensitivity. Software has been incorporated into the design such that non-technical personnel can easily perform the assay.
Purpose:
- Designed for use with an integrated DNA extraction and detection program;
- Provides for maximum possible yields of purified DNA;
- Provides highest sensitivity PCR;
- Greatly reduces false negatives.
Features:
- Small footprint: easily transported;
- Field-portable: powered by a single 12-volt battery;
- Rugged design, stable optical configuration;
- Incorporates an analytical algorithm developed by LANL;
- Speed of application: approximately 5 minutes per sample after calibration;
- User-friendly: customized software steps the analyst through the procedure;
- "One instrument-one protocol" design: suitable for laboratory and field use.
Los Alamos can customize a portable DNA quantifier (PDQ) to meet specific customer requirements. A second-generation PDQ has been commissioned by the FBI's Hazardous Material Response Unit and was recently delivered to the FBI.
| Technical POC: |
Peter Stark |
| Program Office Contacts: |
Harold Dogliani Rosalind Newmyer |
Primary Team Members Involved in Research and Development of the Product:
Peter C. Stark: Chemical Science and Technology Division, Los Alamos National Laboratory;
Cheryl R. Kuske: Life Sciences Division, Los Alamos National Laboratory;
Kenneth I. Mullen: Environment, Safety and Health Division, Los Alamos National Laboratory.
Operated by the